The module covers a selection of advanced instrumental spectroscopic and chromatographic techniques. This module will give the student an understanding of the theoretical basis of chromatography and a comprehensive grounding in the theory, practice and applications of gas chromatography (GC). In addition, the student is introduced to high performance liquid chromatography (HPLC) and its applications for forensic, environmental and pharmaceutical analysis.
and background correction, chemical interference, ionisation interference. Applications. Atomic emission spectrometry: flame emission, plasmas, inductively coupled argon plasma, ICP instrumentation, sample introduction, spectrometers (sequential and simultaneous), interferences, quantitation, applications. Molecular Emission: Photoluminescence, photophysical processes, non-radiative processes, effects of molecular structure and solvent, fluorimetry and phosphorimetry, instrumentation, quantitation, interferences, applications (pharmaceutical, forensic, environmental).
Section B - Separation Science: Fundamental theory related to the practice of chromatography. Definition and classification of chromatographic mechanisms, retention factor, selectivity, column efficiency, band broadening and resolution. Chromatographic modes (reverse phase, normal phase) and applications. GC separation modes, carrier gases and stationary phases, packed and capillary columns and sample introduction. Principles of operation of selected detectors (FID, TCD, ECD). Sample Preparation: Programmed temperature GC, , purge and trap, thermal desorption and headspace analysis. Solid phase microextraction HPLC: instrumentation used, pumps, columns, detectors (UV, PDA, RI, conductivity). Quantitative methods used in chromatography. Selected applications from USP/BP monographs and environmental and forensic analysis.
Laboratory Work: Laboratory work which complements the lecture material is sourced from the School of Chemical and Pharmaceutical Sciences Laboratory Manual.
Spectroscopy
Atomic Absorption Spectroscopy (AAS) instrumentation and applications. Sample preparation and analysis including chemical and ionisation interferences.Atomic emission spectrometry: flame emission, plasmas, inductively coupled argon plasma, ICP instrumentation, sample introduction, spectrometers (sequential and simultaneous), interferences, quantitation, applications. Molecular Emission: Photoluminescence, photophysical processes, non-radiative processes, effects of molecular structure and solvent, fluorimetry and phosphorimetry, instrumentation, quantitation, interferences, applications (pharmaceutical, forensic, environmental).
Chromatography
Fundamental theory related to the practice of chromatography. Definition and classification of chromatographic mechanisms, retention factor, selectivity, column efficiency, band broadening and resolution. Chromatographic modes (reverse phase, normal phase) and applications. GC separation modes, carrier gases and stationary phases, packed and capillary columns and sample introduction. Principles of operation of selected GC detectors (FID, TCD, ECD) and GC/MS. Sample Preparation: Programmed temperature GC, purge and trap, thermal desorption and headspace analysis. Solid phase microextraction (SPME) HPLC: instrumentation used, pumps, columns, detectors (UV, PDA, RI, conductivity). Quantitative methods used in chromatography. Selected applications from USP/BP monographs and environmental and forensic analysis.
Laboratory
Laboratory work which complements the lecture material is sourced from the School of Chemical and BioPharmaceutical Sciences Laboratory Manual.
Delivery by means of lectures (20 hours), tutorials (4 hours), laboratory practicals (15 hours) and self-study (61 hours) to include computational problems and report writing.
| Module Content & Assessment | |
|---|---|
| Assessment Breakdown | % |
| Formal Examination | 70 |
| Other Assessment(s) | 30 |